王珠银 博士、教授、博士生导师 电话：+86-931-8912263 邮箱： zywang@lzu.edu.cn

•教育背景、工作经历

2017-至今            兰州大学，功能有机分子化学国家重点实验室，教授。

2014-2016           河海大学，力材学院教授，博导。

2013-2016           南京基诺美生物科技有限公司，总经理

2010-2013           南京金斯瑞生物科技有限公司，副总裁，兼新技术开发部总监。

2007-2009           美国GenScript 公司，研发部总监。

2006-2007           美国GenScript 公司，蛋白部总监

2004-2006           美国GenScript 公司，主任研究员

1999-2004           美国PharmaSeq, Inc.，高级科学家

1998-1999           哥伦比亚大学（导师: David J. Volsky），博士后

1993-1998           罗格斯大学（导师: Tariq M. Rana），理学博士

1987-1990           兰州大学化学系，理学硕士

1982-1987           兰州大学化学系，理学学士

•荣誉奖励

2011                   入选江苏省“双创计划”

2009                   荣获美国 GenScript公司颁发的“创新奖”

1997                   荣获美国罗格斯大学“Stanley Mandeles 研究奖”

•研究兴趣

高通量药物筛选，合成生物学，蛋白质和多肽，多肽药物

•代表成果

论文：

1. Effects of  human immunodeficiency virus type 1 on astrocyte gene expression and function: Potential role in neuropathogenesis. Zhuying Wang, Gusta Trillo Pazos, Seon Young Kim, Mario Canki, Susan Morgello, Leroy R. Sharer, Harris A. Gelbard, Zaozhong Su, Dongchul Kang, Andrew I. Brooks, Paul B. Fisher, and David J. Volsky*. J. NeuroVirology 2004, 10 (Suppl.1)1-8. 

2. Reduced expression of glutamate transporter EAAT2 and impaired glutamate transport in human primary astrocytes exposed to HIV-1 or gp 120. Zhuying Wang, Olga Pekarskaya, Meryem Bencheikh, Wei Chao, Harris A. Gelbard, Anuja Ghorpade, Jeffrey D. Rothstein, and David J. Volsky*. Virology  2003, 312(1), 60-73.

3. Probing Tat Peptide-TAR Interactions by Psoralen Photo-Cross-Linking. Zhuying Wang, Kavita Shah and Tariq M. Rana*, Biochemistry 2001, 40, 6458-6464.

4. Probing RNA-Protein Interaction by Psoralen photocrosslinking. Zhuying Wang and Tariq M. Rana*, Methods Mol. Biol. 1999, 118, 49-62.

5.  Proximity of a Tat peptide to the HIV-1 TAR RNA loop region determined by Site-Specific Photo-Cross-linking. Zhuying Wang and Tariq M. Rana*. Bioconjugate Chemistry 1999, 10, 512-519. 

6. RNA-Protein Interactions in the Tat-TAR Complex Determined by Site-Specific Photocrosslinking. Zhuying Wang and Tariq M. Rana*, Biochemistry 1998, 37, 4235-4243.

7. DNA Damage-Dependent Transcriptional Arrest and Termination of RNA Polymerase II Elongation Complexes in DNA Template Containing HIV-1 Promoter. Zhuying Wang and Tariq M. Rana*, Proc. Natl. Acad. Sci. USA, 1997, 9, 6688-6693. 

8. RNA Conformation in Tat-TAR Complex Determined by Site-Specific Photocrosslinking. Zhuying Wang and Tariq M. Rana*, Biochemistry  1996, 35, 6491-6499.

9. Protein Orientation in Tat-TAR Complex Determined by Psoralen Photocrosslinking. Zhuying Wang and Tariq M. Rana*, J. Biol. Chem. 1996, 271, 16995-16998.

10. Chemical Conversion of a TAR RNA-Binding Fragment of HIV-1 Tat Protein into a Site-Specific Crosslinking Agent. Zhuying Wang, Kavita Shah and Tariq M. Rana*, J. Am. Chem. Soc. 1995, 117, 5438-5444.

美国专利：

1. Gel electrophoresis device for loading large sample. Patent No.: 9,061,236, Zhuying Wang, Hong Qian, Yuanming Liao and Tao Bai, 2015.

2. Rapid electrophoresis binding method and related kits and compositions. Patent No.: 8,968,541, Zhuying Wang, Jinyu Yang, Fanli Meng, Bing Lai, Zhan Dai and Fangliang Zhang, 2015.   

3. Homologous recombination-based DNA cloning methods and compositions. Patent No.: 8,815,600, Weiqiang Liu, Ping Yang, Tao Wang, Zhuying Wang, Wenzhu Chen and Fangliang Zhang, 2014. 

4. Metal nanoparticle structures for enhancing fluorescence-based assays. Patent No.: 8,785,352, W. Mandecki, Ji li and Zhuying Wang, 2014.

5. Methods and compositions for enhanced expression and secretion of proteins. Patent No.: 8,603,780, Zhuying Wang and Xiaowu Liu, 2013.

6. Homologous recombination-based DNA cloning compositions. Patent No.: 8,501,454, Weiqiang Liu, Ping Yang, Tao Wang, Zhuying Wang, Wenzhu Chen and Fangliang Zhang, 2013.

7. Composition Related to Rapid ELISA Process. Patent No.: 8,334,103,  Zhuying Wang, Fangliang Zhang and Tao Bai, 2012.

8. Method of Sequence Optimization for improved recombinant protein expression using a particle swarm optimization algorithm. Patent No.:  8,326,547, Xiaowu Liu, Yun He and Zhuying Wang, 2012.

9. Rapid ELISA processes and related compositions". Patent No.: 7,824,867,  Zhuying Wang, Fangliang Zhang and Tao Bai, 2010.

10.Rapid C-ELISA process and related compositions". Patent No.: 7,816,092,  Zhuying Wang, Fangliang Zhang and Tao Bai, 2010.

11. Apparatus and method for detecting genetic mutations". Patent No.: 7,279,280,  Michael Pappas and Zhuying Wang, 2007.